Worksheet Biology Class 12 Chapter 11

 Worksheet     Biology  Class 12        Chapter 11     Name___________________ Roll no. _____

1.      The full form of EFB is ________________________.

2.      The two core techniques that enabled birth of modern biotechnology are:

_____________________________ and __________________________

3.      The techniques of genetic engineering include creation of rec_________________________ , use of gene ___________________ and gene __________________.

4.      ___________________________ are also called molecular scissors.  

5.      The plasmid acts as ______________ to transfer the piece of DNA attached to it.

6.      The three basic steps in genetically modifying an organism are:

(i)                 Identification of ________ with desirable __________

(ii)               Introduction of the indentified _______ into the _________

(iii)             Maintenance of introduced ______ in the _______ and _________ of the ________ to its progeny.

7.      Name any two restriction enzymes: ______________________and ____________________

8.      In gel electrophoresis, DNA fragment is stained in _________________ and is exposed to ________ light.

9.      The process of separating and extracting of DNA fragments from the gel piece by washing with chemicals is called __________________.

10.  Origin ______________________ or ori is a sequence of from where __________________ starts.

11.  Ampicillin resistance gene and Tetracycline resistance gene are considered useful ___________________.

12.  Micro-injection and gene gun are used to introduce ______________ into the host cells.

13.  The full form of EFB is ________________________.

14.  The two core techniques that enabled birth of modern biotechnology are:

_____________________________ and __________________________

15.  The techniques of genetic engineering include creation of _________________________ , use of _____________________ and ____________________.

16.  ___________________________ are also called molecular scissors.  

17.  The plasmid acts as ______________ to transfer the piece of DNA attached to it.

18.  The three basic steps in genetically modifying an organism are:

(i)                 ______________________________________________

(ii)               _____________________________________________

(iii)             ________________________________________________________________________

____________________________.

19.  Name any two restriction enzymes: ______________________and ____________________

20.  In gel electrophoresis, DNA fragment is stained in _________________ and is exposed to ________ light.

21.  ________________________________________________________________________________________________________________________________________is called elution.

22.  Origin ______________________ or ori is ______________________________________________.

23.  Ampicillin resistance gene and Tetracycline resistance gene are _____________________________

_________________________.

24.  Micro-injection and gene gun are ____________________________________________________.  

25.  Draw a diagrammatic representation of recombinant DNA technology.